B. Denaturation of Proteins Treatment Observations Reason for Changes 1. Heat clear/cloudy denaturation 2. Acid Cloudy appearance Protein was denatured 3. Base No cloudy appearance/ looks same No change in the protein 4. Alcohol Semi cloudy, a bit clear Hydrophobic interactions 5. Heavy metal ions cloudy , turned into a white color Interaction between COO and NH Questions and Problems 1. Why are heat and alcohol used to disinfect medical equipment? Heat and alcohol are used to disinfect medical